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pcmv3 vector backbone  (Sino Biological)


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    Structured Review

    Sino Biological pcmv3 vector backbone
    Pcmv3 Vector Backbone, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv3+vector+backbone/pCMV3-untagged+Negative+Control+Vector/pm40159502-211-21-24
    Average 95 stars, based on 130 article reviews
    pcmv3 vector backbone - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Expressing:

    Article Title: Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2
    Article Snippet: GCase wt was expressed using a commercially available plasmid (pCMV3-GBA-wt, Sino Biological Inc., Peking, China, #HG12038-UT). .. The expression construct for the secreted LIMP-2 ectodomain (sLIMP2) was described previously.1 The cDNA comprises amino acids 36-431 of LIMP-2 with an N-terminal IgK leader sequence and a C-terminal, TEV-cleavable 10xHis tag in the pCMV3 vector backbone (Sino Biological Inc., Peking, China, #CV011). .. HEK 293F cells (ThermoFisher Scientific Inc., Waltham, MA, United States, #R79007) were cultivated in FreeStyle 293 expression medium (ThermoFisher Scientific Inc., Waltham, MA, United States, #12338018) in vented flat-bottom Erlenmeyer flasks (ThermoFisher Scientific Inc., Waltham, MA, United States, #4115-0125, #4115-0250, #4115-0500) on a MaxQ CO2 Plus orbital shaker (ThermoFisher Scientific Inc., Waltham, MA, United States, #88881102) at 125 rpm, 37°C and 8% CO2.

    Article Title: Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2
    Article Snippet: GCase wt was expressed using a commercially available plasmid (pCMV3-GBA-wt, Sino Biological Inc., Peking, China, #HG12038-UT). .. The expression construct for the secreted LIMP-2 ectodomain (sLIMP2) was described previously.1 The cDNA comprises amino acids 36-431 of LIMP-2 with an N-terminal IgK leader sequence and a C-terminal, TEV-cleavable 10xHis tag in the pCMV3 vector backbone (Sino Biological Inc., Peking, China, #CV011). .. HEK 293F cells (ThermoFisher Scientific Inc., Waltham, MA, United States, #R79007) were cultivated in FreeStyle 293 expression medium (ThermoFisher Scientific Inc., Waltham, MA, United States, #12338018) in vented flat-bottom Erlenmeyer flasks (ThermoFisher Scientific Inc., Waltham, MA, United States, #4115-0125, #4115-0250, #4115-0500) on a MaxQ CO2 Plus orbital shaker (ThermoFisher Scientific Inc., Waltham, MA, United States, #88881102) at 125 rpm, 37°C and 8% CO2.

    Construct:

    Article Title: Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2
    Article Snippet: GCase wt was expressed using a commercially available plasmid (pCMV3-GBA-wt, Sino Biological Inc., Peking, China, #HG12038-UT). .. The expression construct for the secreted LIMP-2 ectodomain (sLIMP2) was described previously.1 The cDNA comprises amino acids 36-431 of LIMP-2 with an N-terminal IgK leader sequence and a C-terminal, TEV-cleavable 10xHis tag in the pCMV3 vector backbone (Sino Biological Inc., Peking, China, #CV011). .. HEK 293F cells (ThermoFisher Scientific Inc., Waltham, MA, United States, #R79007) were cultivated in FreeStyle 293 expression medium (ThermoFisher Scientific Inc., Waltham, MA, United States, #12338018) in vented flat-bottom Erlenmeyer flasks (ThermoFisher Scientific Inc., Waltham, MA, United States, #4115-0125, #4115-0250, #4115-0500) on a MaxQ CO2 Plus orbital shaker (ThermoFisher Scientific Inc., Waltham, MA, United States, #88881102) at 125 rpm, 37°C and 8% CO2.

    Article Title: Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2
    Article Snippet: GCase wt was expressed using a commercially available plasmid (pCMV3-GBA-wt, Sino Biological Inc., Peking, China, #HG12038-UT). .. The expression construct for the secreted LIMP-2 ectodomain (sLIMP2) was described previously.1 The cDNA comprises amino acids 36-431 of LIMP-2 with an N-terminal IgK leader sequence and a C-terminal, TEV-cleavable 10xHis tag in the pCMV3 vector backbone (Sino Biological Inc., Peking, China, #CV011). .. HEK 293F cells (ThermoFisher Scientific Inc., Waltham, MA, United States, #R79007) were cultivated in FreeStyle 293 expression medium (ThermoFisher Scientific Inc., Waltham, MA, United States, #12338018) in vented flat-bottom Erlenmeyer flasks (ThermoFisher Scientific Inc., Waltham, MA, United States, #4115-0125, #4115-0250, #4115-0500) on a MaxQ CO2 Plus orbital shaker (ThermoFisher Scientific Inc., Waltham, MA, United States, #88881102) at 125 rpm, 37°C and 8% CO2.

    Sequencing:

    Article Title: Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2
    Article Snippet: GCase wt was expressed using a commercially available plasmid (pCMV3-GBA-wt, Sino Biological Inc., Peking, China, #HG12038-UT). .. The expression construct for the secreted LIMP-2 ectodomain (sLIMP2) was described previously.1 The cDNA comprises amino acids 36-431 of LIMP-2 with an N-terminal IgK leader sequence and a C-terminal, TEV-cleavable 10xHis tag in the pCMV3 vector backbone (Sino Biological Inc., Peking, China, #CV011). .. HEK 293F cells (ThermoFisher Scientific Inc., Waltham, MA, United States, #R79007) were cultivated in FreeStyle 293 expression medium (ThermoFisher Scientific Inc., Waltham, MA, United States, #12338018) in vented flat-bottom Erlenmeyer flasks (ThermoFisher Scientific Inc., Waltham, MA, United States, #4115-0125, #4115-0250, #4115-0500) on a MaxQ CO2 Plus orbital shaker (ThermoFisher Scientific Inc., Waltham, MA, United States, #88881102) at 125 rpm, 37°C and 8% CO2.

    Article Title: Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2
    Article Snippet: .. The cDNA comprises amino acids 36-431 of LIMP-2 with an N-terminal IgK leader sequence and a C-terminal, TEV-cleavable 10xHis tag in the pCMV3 vector backbone (Sino Biological Inc., Peking, China, #CV011). .. All plasmids in this study were prepared using an endotoxin free maxi prep kit (Macherey-Nagel, Düren, Germany, # 740424.50).

    Article Title: Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2.
    Article Snippet: .. The cDNAcomprises amino acids 36-431 of LIMP-2 with an N-terminal IgK leader sequence and a C-terminal, TEV-cleavable 10xHis tag in the pCMV3 vector backbone (Sino Biological Inc., Peking, China, #CV011). .. All plasmids in this study were prepared using an endotoxin free maxi prep kit (Macherey-Nagel, Düren, Germany, # 740424.50).

    Article Title: Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2
    Article Snippet: GCase wt was expressed using a commercially available plasmid (pCMV3-GBA-wt, Sino Biological Inc., Peking, China, #HG12038-UT). .. The expression construct for the secreted LIMP-2 ectodomain (sLIMP2) was described previously.1 The cDNA comprises amino acids 36-431 of LIMP-2 with an N-terminal IgK leader sequence and a C-terminal, TEV-cleavable 10xHis tag in the pCMV3 vector backbone (Sino Biological Inc., Peking, China, #CV011). .. HEK 293F cells (ThermoFisher Scientific Inc., Waltham, MA, United States, #R79007) were cultivated in FreeStyle 293 expression medium (ThermoFisher Scientific Inc., Waltham, MA, United States, #12338018) in vented flat-bottom Erlenmeyer flasks (ThermoFisher Scientific Inc., Waltham, MA, United States, #4115-0125, #4115-0250, #4115-0500) on a MaxQ CO2 Plus orbital shaker (ThermoFisher Scientific Inc., Waltham, MA, United States, #88881102) at 125 rpm, 37°C and 8% CO2.

    Plasmid Preparation:

    Article Title: Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2
    Article Snippet: GCase wt was expressed using a commercially available plasmid (pCMV3-GBA-wt, Sino Biological Inc., Peking, China, #HG12038-UT). .. The expression construct for the secreted LIMP-2 ectodomain (sLIMP2) was described previously.1 The cDNA comprises amino acids 36-431 of LIMP-2 with an N-terminal IgK leader sequence and a C-terminal, TEV-cleavable 10xHis tag in the pCMV3 vector backbone (Sino Biological Inc., Peking, China, #CV011). .. HEK 293F cells (ThermoFisher Scientific Inc., Waltham, MA, United States, #R79007) were cultivated in FreeStyle 293 expression medium (ThermoFisher Scientific Inc., Waltham, MA, United States, #12338018) in vented flat-bottom Erlenmeyer flasks (ThermoFisher Scientific Inc., Waltham, MA, United States, #4115-0125, #4115-0250, #4115-0500) on a MaxQ CO2 Plus orbital shaker (ThermoFisher Scientific Inc., Waltham, MA, United States, #88881102) at 125 rpm, 37°C and 8% CO2.

    Article Title: Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2
    Article Snippet: .. The cDNA comprises amino acids 36-431 of LIMP-2 with an N-terminal IgK leader sequence and a C-terminal, TEV-cleavable 10xHis tag in the pCMV3 vector backbone (Sino Biological Inc., Peking, China, #CV011). .. All plasmids in this study were prepared using an endotoxin free maxi prep kit (Macherey-Nagel, Düren, Germany, # 740424.50).

    Article Title: Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2.
    Article Snippet: .. The cDNAcomprises amino acids 36-431 of LIMP-2 with an N-terminal IgK leader sequence and a C-terminal, TEV-cleavable 10xHis tag in the pCMV3 vector backbone (Sino Biological Inc., Peking, China, #CV011). .. All plasmids in this study were prepared using an endotoxin free maxi prep kit (Macherey-Nagel, Düren, Germany, # 740424.50).

    Article Title: Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2
    Article Snippet: GCase wt was expressed using a commercially available plasmid (pCMV3-GBA-wt, Sino Biological Inc., Peking, China, #HG12038-UT). .. The expression construct for the secreted LIMP-2 ectodomain (sLIMP2) was described previously.1 The cDNA comprises amino acids 36-431 of LIMP-2 with an N-terminal IgK leader sequence and a C-terminal, TEV-cleavable 10xHis tag in the pCMV3 vector backbone (Sino Biological Inc., Peking, China, #CV011). .. HEK 293F cells (ThermoFisher Scientific Inc., Waltham, MA, United States, #R79007) were cultivated in FreeStyle 293 expression medium (ThermoFisher Scientific Inc., Waltham, MA, United States, #12338018) in vented flat-bottom Erlenmeyer flasks (ThermoFisher Scientific Inc., Waltham, MA, United States, #4115-0125, #4115-0250, #4115-0500) on a MaxQ CO2 Plus orbital shaker (ThermoFisher Scientific Inc., Waltham, MA, United States, #88881102) at 125 rpm, 37°C and 8% CO2.



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